Mouse anti Human CD11a, conjugated with Biotin

• Background:

  CD11a, Leukocyte Function Antigen-1 (LFA-1) complex is a heterodimeric glycoprotein consisting of two non-covalently linked chains, a 180 kDa alpha chain and a 95 kDa beta chain. It is a member of the integrin supergene family of adhesion molecules, and is on virtually all peripheral blood leukocytes. The molecule binds to ICAM-1, 2 and 3 and mediates cell-cell adhesion. This antibody blocks cell-cell adhesion and adhesion mediated events such as cell mediated cytotoxicity.

• Synonyms:

  CD11a Biotin

• UniProt:

  P20701

• Host:

  Mouse

• Species Reactivity:

  Human

• Isotype:

  IgG2a

• Clone:

  OFYS

• Conjugation:

  Biotin

• Type:

  Single-Color Reagent

• Applications:

  Flow Cytometry

• Field of Research:

  Flow Cytometry

• Purification Method:

  Protein A/G Chromatography

• Assay Principle:

  PBMC: Add10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2 to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20ºC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

• Stability:

  See expiration date on vial

• Concentration:

  Titered for flow cytometry

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide and 0,2% carrier protein

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Protein kinase C-mediated regulation of the expression of CD14 and CD11/CD18 in U937 cells. Pedrinaci S; Ruiz-Cabello F; Gomez O; Collado A; Garr Int J Cancer 1990 Feb 1;45(2):294-8 2. Fc gamma and CD11/CD18 receptor expression on normal density and low density Human eosinophils. Hartnell A; Moqbel R; Walsh GM; Bradley B; Kay AB Immunology 1990 Fe;69(2):264-70 3. Leukocyte adhesion molecules deficiency: its structural basis, pathophysiology and implications for modulating the inflammatory response. Arnaout MA Immunol Rev 1990 Ap;114 p145-80 4. Participation of CD11a-c/CD18 and RGD-recognizing adhesion molecules in the binding of LGL to fibroblasts. Evidence for the role of CD11a in the fibroblast-mediated inactivation of NK cells. Scand J Immunol 1990 Au;32(2):137-47 5. Evidence that anti-LFA-1 in vivo improves engraftment and survival after allogeneic bone marrow transplantation. van Dijken PJ; Ghayur T; Mauch P; Down J; Burakoff SJ; Ferrara JL Transplantation 1990 Ma;49(5):882-6 6. Leukocyte-cell adhesion: a molecular process fundamental in leukocyte physiology. Patarroyo M; Prieto J; Rincon J; Timonen T; Lundberg C; Lindbom L; Asjo B; Gahmberg CG Immunol Rev 1990 Ap;114 p67-108 7. Leukocyte Adhesion Deficiency: an inherited defect in Mac-1,LFA-1, and p150,95 glycoproteins Anderson DC and Springer TA, Ann Rev Med 1987;38:175

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  Product should be stored at 4-8ºC; DO NOT FREEZE

• Functional Analysis:

  Flow Cytometry Staining

• CAS Number:

  9007-83-4