Recombinant Human RUNX3 protein (His tag)

Recombinant Human RUNX3 protein (His tag)

• Background: RUNX3, also called CBFA3, AML-2 or PEBP2-alpha C, is a member of the Runt domain family of nuclear transcriptional regulators. All of the RUNX proteins form dimers with CBF-beta. The runt domain (aa 54-186) is required for DNA binding, while a pro/ser/thr-rich region (aa 191-415) transcriptionally activates target genes. Isoform 2 has an alternate 19 aa in place of the N-terminal 5 aa of isoform 1. The 415 aa Human RUNX3 shares 91% aa identity with mouse or rat RUNX3. RUNX3 is necessary for growth control of gastric epithelium, neurogenesis of dorsal root ganglia, and T cell differentiation. RUNX3 expression is frequently mutated in tumors and appears to be silenced by methylation.
• Abbreviation: RUNX3
• Synonyms: Acute myeloid leukemia 2 protein, Core-binding factor subunit alpha-3, Oncogene AML-2, RUNX3, Runt-related transcription factor 3
• CAS Number: 9000-83-3
• UniProt: Q13761
• Accession Number: Q13761
• Host: E. coli
• Origin Species: Human
• Tag: N-His
• Sequence: Met 1-Tyr 415
• Field of Research: Epigenetics and Nuclear signaling;Neuroscience
• Purity: > 95% as determined by reducing SDS-PAGE.
• Bioactivity: Not validated for activity
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
• Molecular Weight: 50 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.