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PNA, Unconjugated

CAT: 0436-LE-6883-10Size: 5 mgDry Ice: NoHazardous: No
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CAT#:0436-LE-6883-10Size:5 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells. Peanut lectin contains one atom of Ca++ and Mg++ per unit.
Other Statements
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
Label
ICT
Type
Lectins
Assay Protocol
Conjugation to Sepharose 4B (solid phase columns) to purify mucin type glycoproteins. For conjugation azide should be removed. Dilute in buffer containing 0.1 mM calcium chloride.
Concentration
5 mg/mL
Shipping Conditions
Ships overnight (domestic), International Priority Shipping
Storage Temperature
2-8°C
Target Description
Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells. Peanut lectin contains one atom of Ca++ and Mg++ per unit.

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