Trypsin Reagent

• Description:

  Trypsin is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

• Specifications:

  This enzyme is supplied as concentrated form along with buffer

• Other Statements:

  For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY

• Label:

  ICT

• Type:

  IHC Reagents

• Applications:

  IHC

• Assay Protocol:

  1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or ImmunoBioScience Universal Immuno buffer. Add endogenous peroxidase block as usual. Wash tissue section 3-5 times with buffer. 2. Remove buffer, add 2-4 drops of ready-to use enzyme solution to cover the tissue section. 3. Incubate at 37oC for 5-10 minutes or 10-15 minutes at room temperature, (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-15 minutes are sufficient at the above temperatures. In some cases it may require more time). 4. Discard trypsin reagent, wash 3-5 times with buffer. 5. The tissue sections are ready for further IHC protocol.

• Components:

  Reagent B (Buffer) 100 ml Reagent E (Enzyme concentrated), 10ml

• Shipping Conditions:

  Ships overnight (domestic), International Priority Shipping

• Storage Temperature:

  2-8 °C; Do not freeze

• Target Description:

  Trypsin is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.