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Trypsin Reagent

CAT: 0436-AR-6541-02Size: 100 mLDry Ice: NoHazardous: No
CAT#:0436-AR-6541-02Size:100 mL
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Trypsin is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.
Description
Trypsin is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
Specifications
This enzyme is supplied as concentrated form along with buffer
Certification
RUO
Other Statements
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
Label
ICT
Type
IHC Reagents
Applications
IHC
Assay Protocol
1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or ImmunoBioScience Universal Immuno buffer. Add endogenous peroxidase block as usual. Wash tissue section 3-5 times with buffer. 2. Remove buffer, add 2-4 drops of ready-to use enzyme solution to cover the tissue section. 3. Incubate at 37oC for 5-10 minutes or 10-15 minutes at room temperature, (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-15 minutes are sufficient at the above temperatures. In some cases it may require more time) . 4. Discard trypsin reagent, wash 3-5 times with buffer. 5. The tissue sections are ready for further IHC protocol.
Components
Reagent B (Buffer) 100 ml Reagent E (Enzyme concentrated), 10ml
Shipping Conditions
Ships overnight (domestic), International Priority Shipping
Storage Conditions
2-8 °C; DO NOT FREEZE
Storage Temperature
2-8 °C; Do not freeze
Target Description
Trypsin is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.

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