VEGFR-2/KDR, Antagonistic

• Description:

  VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs) . All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. Human VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis. The antibody will bind near the ligand binding site of the receptor and has antagonistic activity by blocking the binding of natural ligands.

• Synonyms:

  Vascular endothelial growth factor receptor-2 ; Kdr; Ly73; Flk-1; VEGFR2; VEGF receptor 2; kinase insert domain protein receptor

• NCBI Gene ID:

  16542

• UniProt:

  P35918

• Accession Number:

  ACJ66293.1

• Accession Number mRNA:

  EU884114

• Chromosomal Location:

  5 C3.3; 5 42.0 cM

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse

• Target Antigen:

  Mouse VEGFR2 extracellular domain

• Clone:

  (#MAB0701)

• Applications:

  WB, Attenuation of VEGF-induced KDR tyrosine phosphorylation, IP

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge the vial prior to opening. Reconstitute the antibody with 200 µl sterile PBS and the final concentration is 500 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS (sterile)

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Rat

• Isotype:

  IgG1