VEGFR-2/KDR, Agonistic

• Description:

  VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs) . All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. Human VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis. The antibody will bind near the ligand binding site of the receptor and has agonistic activity.

• Synonyms:

  Vascular endothelial growth factor receptor-2 ; KDR; FLK1; CD309; VEGF receptor 2; kinase insert domain protein receptor

• NCBI Gene ID:

  3791

• UniProt:

  P35968

• Accession Number:

  NP_002244.1

• Accession Number mRNA:

  NM_002253.2

• Chromosomal Location:

  4q11-q12

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  HVEGFR2 N-terminal fragment (N30-200)

• Clone:

  (#8A01)

• Applications:

  WB, IP, Activation of VEGFR-2

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge the vial prior to opening. Reconstitute the antibody with 200 µl sterile PBS and the final concentration is 500 µg/ml.

• Bioactivity:

  VEGFR2 activation: This antibody can induce hVEGFR2 phosphorylation in HUVECs at > 5.0 μg/mL. Western blot: Yes. Immunoprecipitation: Yes.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS (sterile)

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Mouse

• Isotype:

  IgG1