VEGF-E

CAT: 0209-300-044Size: 5 µgDry Ice: NoHazardous: No
CAT#:0209-300-044Size:5 µg
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Description
A DNA sequence encoding the mature variant of ov-VEGF-E isolate D1701 (Dehio et al., 1999; GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994) . Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight Cysteine residues of the central Cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins (Dehio et al., 1999; Wise et al., 1999) . Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E cannot bind to VEGF receptor-1 (Flt-1) . Furthermore VEGF-E can also not bind to VEGF receptor-3 (FLT-4) . Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor –2/KDR.
Synonyms
Vascular Endothelial Growth Factor-E
UniProt
Q9YMF3
Accession Number
AAD03735.1
Accession Number mRNA
AF106020.1
Reactivity
Orf virus
Cross Reactivity
Orf Virus
Sequence
MGSSHHHHHHSSGLVPRGSHDSTKTWSEVFENSGCKPRPMVFRVHDEHPELTSQRFNPPCVTLMRCGGCCNDESLECVPTEEANVTMQLMGASVSGGNGMQHLSFVEHKKCDCKPPLTTTPPTTTRPPRRRR
Assay Protocol
The lyophilized ovVEGF-E should be reconstituted in water or medium to a concentration not lower than 50 µg/ml. For long term storage we would recommend to add at least 0.1% human or bovine serum albumin.
Endotoxin
< 0.1 ng per µg of ov-VEGF-E
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using primary HUVECs. The ED50 for this effect is typically 1 – 5 ng/mL.
Length
132
Form
Lyophilized
Buffer
PBS
Reconstitution
Water
Molecular Weight
35 kDa (Dimer)
Host or Source
E. coli

Related Products

CatalogName
600-309-L50VEGF 183 / VEGF-A Protein
600-309-L20VEGF 183 / VEGF-A Protein
102-P91GVEGF-A
103-PABi03VEGF-A
101-M58VEGF-A
103-PA03AGVEGF-A
104-P15VEGF-A
101-M57VEGF-A
101-M855VEGF-A
101-MBi60VEGF-A

Alternative Products

CatalogName
OPPA00528-2UGVEGF E Protein
MBS691797-01Orf Virus VEGF-E
OM301377-01VEGF E (Orf Virus)
sc-374628VEGF-C (E-6)
sc-390741EG-VEGF (E-12)
DA3516SVEGF-E Orf Virus Protein
sc-374628 FITCVEGF-C (E-6) FITC
sc-390741 ACEG-VEGF (E-12) AC
sc-374628 HRPVEGF-C (E-6) HRP
sc-374628 PEVEGF-C (E-6) PE

Popular Products