VEGF-A

• Description :

  Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF) or vasculotropin, is a homodimeric 34 - 42 kDa, heparin-binding glycoprotein with potent angiogenic, mitogenic and vascular permeability-enhancing activities specific for endothelial cells. Different isoforms can be generated by differential splicing (e.g. VEGF165) . All eight cysteine residues involved in intra- and inter-chain disulfide bonds are conserved among these growth factors. A cDNA encoding a protein having a 53% amino acid sequence homology in the PDGF-like region of VEGF has been isolated from a human placental cDNA library. This protein, named placenta growth factor (PlGF), is now recognized to be a member of the VEGF family of growth factors. Two receptor tyrosine kinases have been described as putative VEGF receptors. Flt-1 (fms-like tyrosine kinase), and KDR (kinase-insert-domain-containing receptor) proteins have been shown to bind VEGF-A with high affinity. In vitro, VEGF is a potent endothelial cell mitogen. The antibody is against the N-terminus and will recognize all VEGF-A isoforms.

• Synonyms :

  VEGFA; VPF; VEGF; MVCD1

• NCBI Gene ID :

  7422

• UniProt :

  P15692

• Accession Number :

  NP_001165097

• Accession Number mRNA :

  NM_001171626

• Chromosomal Location :

  6p12

• Reactivity :

  Anti-Human

• Cross Reactivity :

  Human

• Target Antigen :

  Recombinant human VEGF165 (RT #300-036)

• Clone :

  Rabbit IgG

• Applications :

  WB, IP

• Purification Method :

  Protein A purified

• Assay Protocol :

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity :

  IP: Use 1-5 µg/ml

• Form :

  Lyophilized

• Buffer :

  PBS

• Reconstitution :

  Water

• Storage Conditions :

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Host or Source :

  Rabbit