VEGF-C

• Description:

  VEGF-C, a member of the VEGF/PDGF family of structurally related proteins, is a potent angiogenic cytokine. It promotes endothelial cell growth, promotes lymphangiogesis, and can also affect vascular permeability. VEGF-C is expressed in various tissues, but is not produced in peripheral blood lymphocytes. It forms cell surfaced-associated non-covalent disulfide linked homodimers, and can bind and activate both VEGFR-2 (flk1) and VEGFR-3 (flt4) receptors. During embryogenesis, VEGF-C may play a role in the formation of the venous and lymphatic vascular systems. Both VEGF-C and VEGF-D are over-expressed in certain cancers, and the resulting elevated levels of VEGF-C or VEGF-D tend to correlate with increased lymphatic metastasis. Recombinant, fully processed human VEGF-C is a 13.5 kDa non-disulfide linked homodimeric protein consisting of two 116 amino acid polypeptide chains. Due to glycosylation the protein migrates as a 20.0-22.0 kDa band under non-reducing condition.

• Synonyms:

  Vascular endothelial growth factor C; VEGFC; VRP; Flt4-L

• NCBI Gene ID:

  7424

• UniProt:

  P49767

• Accession Number:

  NP_005420.1

• Accession Number mRNA:

  NM_005429.2

• Chromosomal Location:

  11q13

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Recombinant human VEGF-C

• Clone:

  (#2E65)

• Applications:

  WB, IHC (P)

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Bioactivity:

  WB: 1:1000; IHC (Formalin/Paraffin) : 1:25-50

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Mouse

• Isotype:

  IgG2