VEGF-A

• Description:

  Mouse VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant murine VEGF (isoform 164) is a 39.0 kDa disulfide-linked homodimeric protein consisting of two 164 amino acid polypeptide chains.

• Synonyms:

  Vegfa; Vpf; Vegf; Vegf120; Vegf164; Vegf188

• NCBI Gene ID:

  22339

• UniProt:

  Q00731

• Accession Number:

  NP_001020421

• Accession Number mRNA:

  NM_001025250

• Chromosomal Location:

  17C; 17 24.2cM

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse, Human

• Target Antigen:

  Recombinant mouse VEGF164 (RT #M30-001)

• Clone:

  Rabbit IgG

• Label:

  Biotin

• Applications:

  E

• Purification Method:

  Antigen-affinity purified

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  ELISA: use at 1-10 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Additives:

  BSA (50x), 0.02% sodium azide

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Host or Source:

  Rabbit