VEGF-A, Blocking Antibody

• Description:

  Mouse VEGF (or VEGF-A) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant murine VEGF (isoform 164) is a 39.0 kDa disulfide-linked homodimeric protein consisting of two 164 amino acid polypeptide chains. The antibody blocks the binding of VEGF to VEGFR-2 (mouse FLK) and therefore the initiation of signal transduction.

• Synonyms:

  Vegfa; Vpf; Vegf; Vegf120; Vegf164; Vegf188

• NCBI Gene ID:

  22339

• UniProt:

  Q00731

• Accession Number:

  NP_001020421

• Accession Number mRNA:

  NM_001025250

• Chromosomal Location:

  17C; 17 24.2cM

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse

• Target Antigen:

  Recombinant mouse VEGF

• Clone:

  (#MAB0705)

• Applications:

  N, WB

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge the vial prior to opening. Reconstitute the antibody with 200 µl sterile PBS and the final concentration is 500 µg/ml.

• Bioactivity:

  WB (mouse/human) ; Neutralization of VEGF: human, mouse

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS (sterile)

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Mouse

• Isotype:

  IgG1