VEGF120

CAT: 0209-R20-063Size: 5 µgDry Ice: NoHazardous: No
CAT#:0209-R20-063Size:5 µg
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Description
Rat Vascular Endothelial Growth Factor120 (VEGF120), a 14.1 kDa protein consisting of 120 amino acid residues, is produced as a homodimer. VEGF120 is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF120 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (Flk-1) . Consistent with the endothelial cell-specific action of VEGF120, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extravillous trophoblasts. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo and is also a chemo attractant for monocytes and endothelial cells. At least four different proteins are generated by differential splicing of the mouse VEGF gene: VEGF120, VEGF144, VEGF164 and VEGF188. The most abundant form is VEGF164. Whereas VEGF120, VEGF144 and VEGF164 are secreted proteins, VEGF188 is strongly cell-associated. In addition, the isoforms VEGF164 and VEGF188 bind to heparin with high affinity. All dimeric forms possess similar biological activities. A related protein of VEGF is placenta growth factor (PlGF) with about 53% homology and VEGF-B with similar biological activities. The full ORF of native rat VEGF120 (Ala27-Arg146) was cloned from total RNA of rat sinusoidal endothelial cells using standard protocols.
Synonyms
Vascular Endothelial Growth Factor A; Vegfa; Vegf; VEGF120
NCBI Gene ID
83785
UniProt
P16612
Accession Number
NP_114024.2.
Accession Number mRNA
NM_031836.2
Reactivity
Rat
Cross Reactivity
Rat
Sequence
APTTEGEQKAHEVVKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNVTMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKCDKPRR
Assay Protocol
The lyophilized VEGF120 should be reconstituted in ddH2O to a concentration not lower than 50µg/ml.
Purity
> 90% by SDS-PAGE
Bioactivity
Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 2-10 ng/ml.
Length
120
Form
Lyophilized (freeze-dried)
Buffer
PBS
Reconstitution
Water
Molecular Weight
14.02 kDa
Storage Conditions
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted VEGF120 should be stored in working aliquots at -20°C.
Host or Source
E. coli
N Terminal Sequence
APTTEGEQKAH

Related Products

CatalogName
300-031VEGF121
300-072-L100VEGF121
300-072VEGF121
300-031SVEGF121
300-032VEGF121
300-032-L100VEGF121
300-071VEGF121
300-071SVEGF121
600-310-L50VEGF110 / VEGF-A Protein
300-076-L50VEGF165

Alternative Products

CatalogName
MBS691724-01Mouse VEGF120
MBS691696-01Rat VEGF120
HY-P72775-01VEGF120, Mouse
PRP1134-01Mouse VEGF120 Protein
4964-10VEGF120, murine recombinant
P6119-500μgRecombinant Murine VEGF120
P1180-.5Recombinant Mouse VEGF120, Yeast
AR31053PU-NVEGF-A (VEGF120) Rat Protein
NBP2-76336-100ugRecombinant Rat VEGF120 Protein
NBP2-35188-10ugRecombinant Mouse VEGF120 Protein

Popular Products