TSG (Twisted gastrulation)

• Description:

  Twisted gastrulation (TSG) is a secreted, cysteine-rich protein that plays a role in dorsal/ventral patterning in Drosophila and Xenopus by regulating BMP signaling. TSG was originally identified in Drosophila melanogaster and shown to be required for the differentiation of the dorsal amnioserosa cells. Studies of expression and function of Twisted Gastrulation have been performed in Drosophila and Xenopus. Xenopus TSG is expressed in the ventral regions of the embryo during gastrulation, mimicking the BMP-4 expression pattern. TSG is expressed in dorsal cells of the blastoderm embryo, where there are also high levels of activity of Dpp and Screw. Vertebrate TSGs were subsequently cloned in mouse, human, zebrafish and frog. TSG acts as an agonist for BMP signaling by modulating the inhibitory actions of the BMP antagonist, Chordin/Sog, and the cleavage properties of the metalloprotease, xolloid/tolloid. The N-terminal domain of TSG can bind BMP protein directly in vitro and shows BMP antagonist activity.

• Synonyms:

  Twsg1; Tsg; Twg; AW552143; D17Ertd403e; 1810013J15Rik; 9030422N06Rik

• NCBI Gene ID:

  65960

• UniProt:

  Q9EP52

• Accession Number:

  NP_075540.1

• Accession Number mRNA:

  NM_023053.3

• Chromosomal Location:

  17 E1.1; 17 38.0 cM

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse

• Target Antigen:

  Mouse recombinant protein of TSG

• Clone:

  (#7D19)

• Applications:

  WB, IHC (P)

• Purification Method:

  Protein A/G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Rat

• Isotype:

  IgG2