TIMP-2 Recombinant Protein

• Description:

  TIMP-2 is an extracellular inhibitor of MMPs including MMP1, 2, 3, 7, 8, 9, 10, 12, 13, 14, 15, 16 and 19. It belongs to the I35 (TIMP) family of irreversible protease inhibitors that function as key modulators of extracellular matrix degradation during tissue development and remodeling. TIMP-2 can also act through an MMP-independent mechanism inhibiting endothelial cell proliferation in vitro and demonstrates anti-angiogenic activities in vivo. Recombinant human TIMP-2 is a 21.8 kDa protein containing 194 amino acid residues.

• Synonyms:

  Tissue inhibitor of metalloproteinases 2, CSC-21K

• CAS Number:

  9000-83-3

• NCBI Gene ID:

  7077

• UniProt:

  P16035

• Accession Number:

  NP_003246

• Accession Number mRNA:

  NM_003255

• Gene Location:

  17q25

• Host:

  E. coli

• Origin Species:

  Human

• Species Reactivity:

  Human

• Sequence:

  CSCSPVHPQQ AFCNADVVIR AKAVSEKEVD SGNDIYGNPI KRIQYEIKQI KMFKGPEKDI EFIYTAPSSA VCGVSLDVGG KKEYLIAGKA EGDGKMHITL CDFIVPWDTL STTQKKSLNH RYQMGCECKI TRCPMIPCYI SSPDECLWMD WVTEKNINGH QAKFFACIKR SDGSCAWYRG AAPPKQEFLD IEDP

• Detection Range:

  TIMP-2 activity was measured by its ability to inhibit human MMP-1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP-2 concentration of approximately 0.5 μg/ml, when using an MMP-1 concentration of 1.6 μg/ml.

• Endotoxin:

  < 0.1 ng/µg of protein (<1EU/µg)

• Purity:

  > 95% by SDS-PAGE & HPLC analyses

• Length:

  194

• Form:

  Lyophilized

• Molecular Weight:

  21.8 kDa

• Shipping Conditions:

  Room Temperature