SCGF-beta

• Description:

  SCGF-α and -β are hematopoietic growth factor that exerts their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively. Recombinant human SCGF-β is a 25.0 kDa polypeptide containing 227 amino acid residues.

• Synonyms:

  CLEC11A; P47; SCGF; LSLCL; CLECSF3

• NCBI Gene ID:

  6320

• UniProt:

  Q9Y240

• Accession Number:

  NP_002966.1

• Accession Number mRNA:

  NM_002975.2

• Chromosomal Location:

  19q13.3

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  MGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN CVAQASDDGS WWDHDCQRRL YYVCEFPF

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells, previously grown in media containing GM-CSF. The expected ED50 for this effect is ≤ 7.0 ng/ml.

• Length:

  227

• Form:

  Lyophilized

• Molecular Weight:

  25.0 kDa

• Host or Source:

  E. coli