Mouse Anti-Human SCF Monoclonal Antibody

Mouse Anti-Human SCF Monoclonal Antibody

• Description: Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. The ECD shows both N-linked and O-linked glycosylation. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice. An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within the ECD of the short isoform (corresponding to this recombinant protein), human SCF shares 75 - 83% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF-R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.
• Synonyms: KITLG; SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7; kit-ligand
• CAS Number: 9007-83-4
• NCBI Gene ID: 4254
• UniProt: P21583
• Accession Number: NP_000890
• Accession Number mRNA: NM_000899
• Gene Location: 12q22
• Host: Mouse
• Origin Species: Human
• Species Reactivity: Human
• Target Antigen: Recombinant Human SCF
• Isotype: IgG1
• Validated Applications: ELISA
• Purification Method: Protein A chromatography
• Form: Lyophilized from PBS
• Buffer: PBS
• Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
• Shipping Conditions: Room Temperature
• Storage Conditions: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.