Mouse Anti-Human ErbB2 Monoclonal Antibody

Mouse Anti-Human ErbB2 Monoclonal Antibody

• Description: ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be overexpressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGF R), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3 kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with betacatenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage.
• Synonyms: ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
• CAS Number: 9007-83-4
• NCBI Gene ID: 2064
• UniProt: P04626
• Accession Number: NP_004439.2
• Accession Number mRNA: NM_004448.2
• Gene Location: 17q11.2-q12; 17q21.1
• Host: Mouse
• Origin Species: Human
• Species Reactivity: Human
• Target Antigen: Recombinant Human ErbB2 protein
• Isotype: IgG2
• Clone: (#1K11)
• Validated Applications: WB, IHC (P)
• Purification Method: Protein G chromatography
• Form: Lyophilized
• Buffer: PBS
• Reconstitution: Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
• Shipping Conditions: Room Temperature
• Storage Conditions: Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.