Mouse Anti-Human Betacellulin Monoclonal Antibody

Mouse Anti-Human Betacellulin Monoclonal Antibody

• Description: Betacellulin (BTC) is a new member of the EGF family of cytokines that is comprised of at least ten proteins including EGF, TGF αlpha, amphiregulin, HBEGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or more EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. BTC, a heparin binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotein composed of 80 amino acid residues. The cDNA encoding human BTC was cloned from a human breast adenocarcinoma cell line (MCF7) cDNA library. Human and mouse cDNAs encode BTC precursor proteins of 178 and 177 amino acid residues, respectively. At the amino acid sequence level, human BTC precursor protein exhibits 79% identity with that of the mouse BTC precursor. In a mouse cell line transfected with human BTC cDNA, three forms of soluble human BTC have been detected: the glycosylated, intact BTC composed of 80 amino acid residues, a truncated molecule lacking 12 amino acid residues from the amino terminus, and a second truncated molecule lacking 30 amino acid residues from the amino terminus. The biological activities of these BTC forms were shown to be identical. BTC can bind to the EGF receptor and is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells.
• Synonyms: BTC
• CAS Number: 9007-83-4
• NCBI Gene ID: 685
• UniProt: P35070
• Accession Number: NP_001720
• Accession Number mRNA: NM_001729
• Gene Location: 4Q13-q21
• Host: Mouse
• Origin Species: Human
• Species Reactivity: Human
• Target Antigen: Recombinant Human Betacellulin
• Isotype: IgG1
• Clone: (#6G23)
• Validated Applications: Capture Ab
• Purification Method: Protein G chromatography
• Form: Lyophilized
• Buffer: PBS
• Reconstitution: Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
• Shipping Conditions: Room Temperature
• Storage Conditions: Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.