Resistin antagonist

• Description:

  Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins (monomeric peptide contains 11 cysteine residues) referred to as the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) or FIZZ3 (Found in Inflammatory Zone 3) . Human resistin antagonist is a monomeric C7A mutant that does not form covalent dimers. It is expressed and purified by chromatographic techniques.

• Synonyms:

  Adipose tissue-specific secretory factor (ADSF), C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3

• NCBI Gene ID:

  56729

• UniProt:

  Q9HD89

• Accession Number:

  NP_065148

• Accession Number mRNA:

  NM_020415

• Reactivity:

  Human

• Cross Reactivity:

  Mouse, Human

• Sequence:

  ASSKTLCSMEE AINERIQEVA GSLIFRAISS IGLECQSVTS RGDLATCPRG FAVTGCTCGS ACGSWDVRAE TTCHCQCAGM DWTGARCCRV QP

• Assay Protocol:

  It is recommended to reconstitute the lyophilized resistin in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other buffered aqueous solutions containing carrier proteins.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  >98.0% as determined by RP-HPLC, SDS-PAGE Silver Stained and Gel filtration.

• Bioactivity:

  The biological activity was evidenced by resistin antagonist activity to inhibit resistin-induced Akt phosphorylation in two cell lines. It also reduced the weight (mainly the visceral fat) and normalized GTT and ITT inHFD-fed mice.

• Length:

  93

• Form:

  Lyophilized

• Reconstitution:

  Water

• Molecular Weight:

  9.7 kDa

• Host or Source:

  E. coli

• N Terminal Sequence:

  ASSKTLA