Prox-1

• Description:

  Prox-1 is a homeobox gene and acts as a master switch for lymphatic endothelial phenotype. Expression of Prox-1 in blood endothelial cells induces expression of other lymphatic marker genes. Together with Podoplanin, Prox-1 can be used to reliably distinguish lymphatic vessels from blood vessels. Prox-1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells. Produced from sera of rabbits immunized with the recombinant highly conserved C-terminal part of the homeobox transcription factor Prox-1.

• Synonyms:

  PROX1; W117m

• NCBI Gene ID:

  5629

• UniProt:

  Q92786

• Accession Number:

  NP_002754.2

• Accession Number mRNA:

  NM_002763.3

• Chromosomal Location:

  1q32.2-q32.3

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Recombinant human Prox-1 (RT #300-052)

• Clone:

  Rabbit IgG

• Applications:

  WB, IF

• Purification Method:

  Antigen-affinity purified

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  Western Blot: Use 1-5 µg/ml

• Form:

  Lyophilized

• Buffer:

  0.5X PBS, pH 7.2

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Host or Source:

  Rabbit