Nidogen-1 (Entactin)

• Description:

  Nidogen-1 (also entactin) is a 150 kDa, secreted, monomeric glycoprotein that serves as a major linking component of basement membranes. It is synthesized as a 1247 amino acid (aa) precursor with a 28 aa signal sequence and a 1219 aa mature protein. The molecule is modular in structure with five distinct regions. Functionally, G1 is reported to bind type IV collagen. The mucin region contains a short peptide that ligates alpha 3 beta 1 integrins. G2 interacts with perlecan, and an RGD motif in the rod-shaped segment serves as a binding site for alpha v beta 3 integrins. Finally, G3 is associated with laminin binding.

• Synonyms:

  Nidogen-1, NID-1, Entactin

• NCBI Gene ID:

  4811

• UniProt:

  P14543

• Accession Number:

  NP_002499.2

• Accession Number mRNA:

  NM_002508.2

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Recombinant Human Nidogen-1/Entactin

• Clone:

  (#2R33)

• Applications:

  EWB, E, IHC

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.

• Host or Source:

  Mouse

• Isotype:

  IgG1