Lyve-1, soluble Recombinant Protein

• Description:

  A DNA sequence encoding the extracellular domain of human LYVE-1 (Met1 to Gly232) was fused to a C-terminal His-tag (6xHis) and expressed in insect cells. Based on N-terminal sequence analysis, the primary structure of recombinant mature sLYVE-1 starts at Ser24. sLYVE-1 has a calculated monomeric molecular mass of about 25 kDa but as a result of glycosylation, migrates at approximately 35 - 45 kDa under reducing conditions in SDS-PAGE. LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

• Synonyms:

  LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1

• NCBI Gene ID:

  10894

• UniProt:

  Q9Y5Y7

• Accession Number:

  NP_006682

• Accession Number mRNA:

  NM_006691

• Gene Location:

  11p15

• Host:

  Insect Cells

• Origin Species:

  Human

• Species Reactivity:

  Human

• Tag:

  His-Tag

• Sequence:

  SLRAEELSIQVSCRIMGITLVSKKANQQLNFTEAKEACRLLGLSLAGKDQVETALKASFETCSYGWVGDGFVVISRISPNPKCGKNGVGVLIWKVPVSRQFAAYCYNSSDTWTNSCIPEIITTKDPIFNTQTATQTTEFIVSDSTYSVASPYSTIPAPTTTPPAPASTSIPRRKKLICVTEVFMETSTMSTETEPFVENKAAFKNEAAGHHHHHH

• Purity:

  > 95% by SDS-PAGE

• Length:

  215

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  The lyophilized sLYVE-1 is soluble in water and most aqueous buffers. The lyophilized sLYVE-1 should be reconstituted in PBS or medium to a concentration not lower than 50µg/ml.

• Molecular Weight:

  ~ 35.0 - 45.0 kDa

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted sLYVE-1 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!

• N Terminal Sequence:

  SLRAEELSI

• CAS Number:

  9000-83-3