Leptin antagonist (mutant L39A/D40A/F41A)

• Description:

  Recombinant mouse leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, recombinant mouse leptin was mutated, resulting in L39A/D40A/F41A antagonist that was purified by proprietary chromatographic techniques. according to Salomon et al (2006) Protein Expression and PuriWcation 47, 128–136.

• Synonyms:

  Lep; ob; obese

• NCBI Gene ID:

  16846

• UniProt:

  P41160

• Accession Number:

  NP_032519.1

• Accession Number mRNA:

  NM_008493.3

• Reactivity:

  Mouse

• Cross Reactivity:

  Mouse

• Sequence:

  AVPIQKVQDDTKTLIKTIVTRINDISHTQSVSAKQRVTGAAAIPGLHPILSLSKMDQTLAVYQQVLTSLPSQNVLQIANDLENLRDLLHLLAFSKSCSLPQTSGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDVSPEC

• Assay Protocol:

  It is recommended to reconstitute the lyophilized recombinant mouse leptin antagonist in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted with other aqueous solutions.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98.0% as determined by Gel filtration and SDS-PAGE gel.

• Bioactivity:

  Recombinant mouse leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.

• Length:

  146

• Form:

  Lyophilized

• Reconstitution:

  Water

• Molecular Weight:

  16.0 kDa

• Host or Source:

  E. coli

• N Terminal Sequence:

  AVPIQ