Leptin antagonist (mutant L39A/D40A/F41A)

• Description:

  Recombinant human leptin is one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Recombinant human leptin was mutated, resulting in L39A/D40A/F41A mutant that was purified by proprietary chromatographic techniques. Preparation of leptin antagonists was recently published in Biochemical Journal (Niv-Spector et al, Biochem. J 291;221-230 (2005) .

• Synonyms:

  Lep; ob; obese

• NCBI Gene ID:

  3952

• UniProt:

  P41159

• Accession Number:

  NP_000221.1

• Accession Number mRNA:

  NM_000230

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  AVPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGAAAIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLENLRDLLHVLAFSKSCHLPWASGLETLDSLGGVLEASGYSTEVVALSRLQGSLQDMLWQLDLSPGC

• Assay Protocol:

  It is recommended to reconstitute the lyophilized recombinant human leptin antagonist in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions preferably in presence of carrier protein.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98.0% as determined by Gel filtration and SDS-PAGE gel.

• Bioactivity:

  Recombinant human leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.

• Length:

  146

• Form:

  Lyophilized

• Reconstitution:

  0.4% NaHCO3, pH 8-9

• Molecular Weight:

  16.0 kDa

• Host or Source:

  E. coli

• N Terminal Sequence:

  AVPIQ