Lactogen, placental

• Description:

  Recombinant ovine placental lactogen, one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques (see Sakal et al. J. Endocrinology (1997), 152, 317-327. Ovine PL is also capable of activating human and other heterologous GH receptors but not ruminat GH receptors.

• Synonyms:

  Chorionic somatomammotropin hormone, Placental lactogen (PL)

• NCBI Gene ID:

  443319

• UniProt:

  P16038

• Accession Number:

  NP_001009309.2

• Accession Number mRNA:

  NM_001009309.4

• Reactivity:

  Ovine

• Cross Reactivity:

  Ovine

• Sequence:

  AQHPPYCRNQPGKCQIPLQSLFDRATTVANYNSKLAGEMVNRFDEQYGQGINSESKVINCHTSSITTPNSKAEAINTEDKILFKLVISLLHSWDEPLHHAVTELANSKGTSPALLTKAQEIKEKAKVLVDGVEVIQKRIHPGEKNEPYPVWSEQSSLTSQDENVRRVAFYRLFHCLHRDSSKIYTYLRILKCRLTSCET

• Assay Protocol:

  It is recommended to reconstitute the lyophilized oPL in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 99.0% as determined by Gel filtration and SDS-PAGE gel.

• Bioactivity:

  Recombinant oPL is fully biologically active as evidenced by inducing proliferation of Nb2 cells.

• Length:

  199

• Form:

  Lyophilized

• Reconstitution:

  Water

• Molecular Weight:

  23.0 kDa

• Host or Source:

  E. coli

• N Terminal Sequence:

  AQHPP