Klotho

• Description :

  The Klotho proteins, Klotho and Klotho beta (beta KL), are type I transmembrane glycoproteins with extracellular regions that contain two beta-glycosidase-like repeats. Klotho has both membrane-bound and soluble forms; the soluble form predominates and consists of only the first beta-glycosidase-like repeat. Klotho has beta-glucuronidase activity against select steroid beta-glucuronides, appears to regulate calcium and vitamin D levels in serum, and is associated with premature aging. Klotho beta, a divergent structural member of the glycosidase I superfamily, is primarily expressed in the liver and pancreas, with lower expression in adipose tissue. Klotho beta mediates the interaction of FGF-15 (human FGF-19) with FGF Receptor 4 in the liver, which negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes. Klotho beta is also a cofactor for the interaction of FGF-21 with FGF Receptor 1c in adipocytes. The expression pattern of Klotho beta suggests it may play a role in lipid metabolism.

• Synonyms :

  Kl; alpha-kl

• NCBI Gene ID :

  16591

• UniProt :

  O35082

• Accession Number :

  NP_038851.2

• Accession Number mRNA :

  NM_013823.2

• Chromosomal Location :

  5; 5 G3

• Reactivity :

  Anti-Mouse

• Cross Reactivity :

  Mouse

• Target Antigen :

  Recombinant mouse Klotho

• Clone :

  (#4F54)

• Applications :

  WB, IHC (F)

• Purification Method :

  Protein A/G chromatography

• Assay Protocol :

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form :

  Lyophilized

• Buffer :

  PBS

• Reconstitution :

  PBS

• Storage Conditions :

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source :

  Rat

• Isotype :

  IgG2