IL-28A

• Description:

  IL-28A is distantly related to type I interferons and the IL-10 family. Expression of IL-28A is induced by viral infection which interacts with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha. IL-28A exhibits common features with type I IFNs such as antiviral activity, antiproliferative activity and in vivo antitumour activity. IL-28A acts similarly to IFNs, but is less effective generally and has activity in a more limited range of cell lines. IFN-ambda 1, IFN-lambda 2 and IFN-lambda 3 are closely positioned genes on human chromosome 19. IL-28A induces ELR (-) CXC chemokine mRNA in human peripheral blood mononuclear cells, in an IFN-gamma-independent manner. IL-28A is able to generate tolerogenic DCs, an activity that could thwart IFN-beta functions. IL-28A produced in response to viral infection, activates both monocytes and macrophages producing a restricted panel of cytokines and therefore is an important factor in activating innate immune responses at the site of viral infection.

• Synonyms:

  Interferon lambda-2, IFN-lambda-2, Interleukin-28A, IL-28A, Ifnl2, Il28a, EG330496

• NCBI Gene ID:

  330496

• UniProt:

  Q4VK74

• Accession Number:

  NP_001019844.2

• Accession Number mRNA:

  NM_001024673.2

• Reactivity:

  Mouse

• Cross Reactivity:

  Mouse

• Sequence:

  DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKDAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENMTDS ALATILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFRLL TRDLKCVASG DQCV

• Assay Protocol:

  It is recommended to reconstitute the lyophilized IL-28A in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 95% by SDS-PAGE & HPLC analyses

• Bioactivity:

  The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis (EMC) virus is less than 3 ng/ml, corresponding to a specific activity of > 3.3 x 105 IU/mg.

• Length:

  174

• Form:

  Lyophilized

• Buffer:

  PBS/5% Trehalose

• Reconstitution:

  Water

• Molecular Weight:

  19.7kDa

• Storage Conditions:

  Lyophilized mouse IL-28A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C.

• Host or Source:

  E. coli