IL-22

• Description:

  IL-22 is a member of the IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes, IL-22 inhibits IL-4 production by Th2 cells, and induces acute phase reactants in the liver and pancreas. IL-22 signals through a receptor system consisting of IL-10R-β/CRF2-4 and IL-22R, both of which are members of the class II cytokine-receptor family. Recombinant murine IL-22 is a 32-34 kDa non-disulfide-linked homodimeric protein containing of two 146 amino acid polypeptide chains.

• Synonyms:

  IL22; IL-22; Iltif; IL-22a; ILTIFa

• NCBI Gene ID:

  50929

• UniProt:

  Q9JJY9

• Accession Number:

  NP_058667.1

• Accession Number mRNA:

  NM_016971

• Chromosomal Location:

  10 D2; 10 67.0 cM

• Reactivity:

  Mouse

• Cross Reactivity:

  Mouse

• Sequence:

  MLPVNTRCKL EVSNFQQPYI VNRTFMLAKE ASLADNNTDV RLIGEKLFRG VSAKDQCYLM KQVLNFTLED VLLPQSDRFQ PYMQEVVPFL TKLSNQLSSC HISGDDQNIQ KNVRRLKETV KKLGESGEIK AIGELDLLFM SLRNACV

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Assay#1: Determined by its ability to activate STAT following receptor ligand interaction. Assay#2: Measured by its ability to induce IL-10 secretion in COLO 205 (human colon carcinoma cells) . The expected ED50 for this effect is 1.0-2.0 ng/ml.

• Length:

  146

• Form:

  Lyophilized

• Molecular Weight:

  33.4 kDa

• Host or Source:

  E. coli