IL-22

• Description:

  IL-22 is a member of the IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes, IL-22 inhibits IL-4 production by Th2 cells, and induces acute phase reactants in the liver and pancreas. IL-22 signals through a receptor system consisting of IL-10R-β/CRF2-4 and IL-22R, both of which are members of the class II cytokine-receptor family. Recombinant human IL-22 is a 33.6 kDa non-disulfide-linked homodimeric protein containing of two 147 amino acid polypeptide chains.

• Synonyms:

  Interleukin-22, IL-TIF, Cytokine ZCYTO18

• NCBI Gene ID:

  50616

• UniProt:

  Q9GZX6

• Accession Number:

  NP_065386.1

• Accession Number mRNA:

  NM_020525.4

• Chromosomal Location:

  12q15

• Reactivity:

  Human

• Cross Reactivity:

  Monkey, Human

• Sequence:

  MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Assay #1: Determined by its ability to stimulate the proliferation of HEK-Blue™ IL-22 reporter cells. Assay #2: Measured by its ability to induce IL-10 secretion in COLO 205 (human colon carcinoma cells) . The expected ED50 for this effect is 0.7- 1.0 ng/ml.

• Length:

  147

• Form:

  Lyophilized

• Molecular Weight:

  33.6 kDa

• Host or Source:

  E. coli