IFN alpha 2b

• Description :

  IFN-alpha is produced by macrophages and has antiviral activities. IFN stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase. IFN-a 2b Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19400 Dalton. The difference between IFNA2A and IFNA2B is in the amino acid present at position 23. IFN-alpha 2a has a lysine at that position 23 while IFN-alpha 2b has arginine. The IFN-alpha 2b gene was obtained from human leukocytes.

• Synonyms :

  IFN alpha 2b, IFNA, INFA2, MGC125764, MGC125765.

• NCBI Gene ID :

  3440

• UniProt :

  P01563

• Accession Number :

  NP_000596-2

• Accession Number mRNA :

  NM_000605-3

• Reactivity :

  Human

• Cross Reactivity :

  Human

• Assay Protocol :

  It is recommended to reconstitute the lyophilized IFN-alpha 2b in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Endotoxin :

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity :

  ≥ 98% by SDS-PAGE gel and HPLC analyses.

• Bioactivity :

  The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 260,000,000 IU/ mg.

• Length :

  166

• Form :

  Lyophilized

• Reconstitution :

  Water

• Molecular Weight :

  19.4 kDa

• Storage Conditions :

  Lyophilized IFN although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN alpha 2b should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .

• Host or Source :

  E. coli

• N Terminal Sequence :

  MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE