ICAM-1 (CD54)

• Description:

  ICAMs are members of the Ig superfamily of calcium-independent transmembrane glycoproteins. ICAM-1 is a ligand for lymphocyte function-associated (LFA) and Mac-1 integrins and the major human rhinovirus receptor. The primary function of ICAM-1 is to provide adhesion between endothelial cells and leukocytes after stress or injury. The human ICAM-1 gene codes for a 505 amino acid transmembrane glycoprotein containing a 29 amino acid cytoplasmic domain, a 23 amino acid transmembrane domain, and a 453 amino acid extracellular domain. Recombinant human ICAM-1 is a 49.5 kDa glycoprotein comprising the extracellular domain (453 amino acid residues) of ICAM-1. Monomeric glycosylated ICAM-1 migrates at an apparent molecular weight of approximately 72.0-80.0 kDa by SDS-PAGE analysis under reducing conditions.

• Synonyms:

  ICAM1; BB2; CD54; P3.58

• NCBI Gene ID:

  3383

• UniProt:

  P05362

• Accession Number:

  NP_000192.2

• Accession Number mRNA:

  NM_000201

• Chromosomal Location:

  19p13.3-p13.2

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Recombinant human ICAM1 N-terminal protein

• Clone:

  (#7D22)

• Applications:

  ELISA, WB, IHC (Formalin/P)

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Bioactivity:

  WB: 1:100-1000; IHC (Formalin/Paraffin) : 1:25-50; ELISA: Yes

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Mouse

• Isotype:

  IgG1