His-tag

• Description :

  The His-tag antibody was generated by immunization of rabbits with several recombinant His-tagged proteins produced in insect cells and E. coli. It was antigen-affinity purified using a His-tagged protein as matrix.

• Synonyms :

  His-tag

• NCBI Gene ID :

  N.d.

• Reactivity :

  Anti-

• Target Antigen :

  Different recombinant His-tag proteins

• Clone :

  Rabbit IgG

• Applications :

  WB

• Purification Method :

  Antigen-affinity purified

• Assay Protocol :

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity :

  Western Blot: Use 1-5 µg/ml

• Form :

  Lyophilized

• Buffer :

  PBS

• Reconstitution :

  Water

• Storage Conditions :

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Host or Source :

  Rabbit