His-tag

• Description:

  The His-tag antibody was generated by immunization of rabbits with several recombinant His-tagged proteins produced in insect cells and E. coli. It was antigen-affinity purified using a His-tagged protein as matrix.

• Synonyms:

  His-tag

• NCBI Gene ID:

  N.d.

• Reactivity:

  Anti-

• Target Antigen:

  Different recombinant His-tag proteins

• Clone:

  Rabbit IgG

• Applications:

  WB

• Purification Method:

  Antigen-affinity purified

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  Western Blot: Use 1-5 µg/ml

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Host or Source:

  Rabbit