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HGFR

CAT: 0209-103-M184Size: 100 µgDry Ice: NoHazardous: No
CAT#:0209-103-M184Size:100 µg
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Description
HGF R, also known as Met (from N-methyl-N-nitro-N-nitroso-guanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfidelinked dimer composed of a 50 kDa extracellular α chain and a 145 kDa transmembrane β chain. The extracellular domain (ECD) contains a seven bladed β-propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like Eset domains, while the cytoplasmic region includes the tyrosine kinase domain. An alternately spliced form of mouse HGF R lacks a cytoplasmic juxtamembrane region important for regulation of signal transduction. The sema domain, which is formed by both the α and β chains of HGF R, mediates both ligand binding and receptor dimerization. Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2containing molecules. HGF stimulation induces HGF R downregulation via internalization and proteasomedependent degradation. In the absence of ligand, HGF R forms noncovalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, integrin α6/β4, plexins B1, 2, 3, and MSP R/Ron (11 18) . Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (11 18) . Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion. Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells. Genetic polymorphisms, chromosomal translocation, overexpression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers. Within the ECD, mouse HGF R shares 87%, 87%, and 94% amino acid sequence identity with canine, human, and rat HGF R, respectively.
Synonyms
Met; HGF; HGFR; Par4; c-Met; AI838057
NCBI Gene ID
17295
UniProt
F8VQL0
Accession Number
NP_032617.2
Accession Number mRNA
NM_008591.2
Chromosomal Location
6 A2; 6 4.0 cM
Reactivity
Anti-Mouse
Cross Reactivity
Mouse
Target Antigen
Recombinant Mouse HGF-R extracellular domain
Clone
(#6F7)
Applications
WB
Purification Method
Protein G chromatography
Assay Protocol
Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Form
Lyophilized
Buffer
PBS
Reconstitution
PBS
Storage Conditions
Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.
Host or Source
Rat
Isotype
IgG1

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