HGF Activator Inhibitor Type 1 (HAI-1)

• Description:

  Hepatocyte growth factor activator inhibitor1 (HAI1) encoded by the Spint1 gene is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGF-A) and matripas. The membraneanchored HAI1 consists of two Kunitz domains, a LDL-recepto-rlike domain, and a C-terminal transmembrane domain. Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface. The shedding of HAI1 and HGFA/HAI1 complex is enhanced by treatment with phorbol 12myristate, 13acetate or IL1β. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor.

• Synonyms:

  Spint1; HAI-1

• NCBI Gene ID:

  20732

• UniProt:

  Q9R097

• Accession Number:

  NP_058603.2

• Accession Number mRNA:

  NM_016907.3

• Chromosomal Location:

  2 E5; 2

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse

• Target Antigen:

  Recombinant mouse HAI-1 extracellular domain

• Clone:

  (#3G21)

• Applications:

  WB

• Purification Method:

  Protein G/A chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Rat

• Isotype:

  IgG2