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HB-EGF

CAT: 0209-102-P65Size: 100 µgDry Ice: NoHazardous: No
CAT#:0209-102-P65Size:100 µg
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PRICE
AVAILABILITY
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HB-EGF is an EGF related growth factor that signals through the EGF receptor, and stimulates the proliferation of smooth muscle cells (SMC), fibroblasts, epithelial cells, and keratinocytes. HB-EGF is expressed in numerous cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes, and certain tumor cells. The ability of HB-EGF to specifically bind heparin and heparin sulfate proteoglycans is distinct from other EGF-like molecules, and may be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells. The human HB-EGF gene encodes a 208 amino acid transmembrane protein, which can be proteolytically cleaved to produce soluble HB-EGF.
Synonyms
HBEGF; DTR; DTS; DTSF; HEGFL
NCBI Gene ID
1839
UniProt
Q99075
Accession Number
NP_001936.1
Accession Number mRNA
NM_001945
Chromosomal Location
5q23
Reactivity
Anti-Human
Cross Reactivity
Human
Target Antigen
Recombinant human HB-EGF (Cat# 100-185)
Clone
Rabbit IgG
Applications
WB, ELISA
Purification Method
Antigen-affinity purified
Assay Protocol
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Bioactivity
To detect Human HB-EGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human HB-EGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. To detect Human HB-EGF by sandwich ELISA (using 100μl/well), a concentration of 0.25-1.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with a Biotinylated Rabbit Anti-Human HB-EGF as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human HB-EGF.
Form
Lyophilized
Reconstitution
Water
Storage Conditions
The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.
Host or Source
Rabbit

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