GDF-5

• Description :

  GDF-5 is expressed in long bones during embryonic development and postnatally in articular cartilage. Mutations in the GDF-5 gene have been implicated in Grebe Syndrome, which is characterized by short stature, extra digits, short and deformed extremities, and in Hunter-Thompson type dwarfism. The mature and functional form of GDF-5 is a homodimer of two 120 amino-acid polypeptide chain (monomers) linked by a single disulfide bond. Each GDF-5 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 27 amino-acid signal peptide and a 348 amino-acid propeptide. This precursor undergoes intracellular dimerization, and upon secretion it is processed by a furin-type protease. Recombinant murine GDF-5 is a 27.0 kDa homodimeric disulfide-linked protein consisting of two 120 amino acids.

• Synonyms :

  Gdf5; bp; brp; Cdmp-1

• NCBI Gene ID :

  14563

• UniProt :

  P43027

• Accession Number :

  NP_032135.2

• Accession Number mRNA :

  NM_008109.2

• Chromosomal Location :

  2 H1; 2 90.0 cM

• Reactivity :

  Anti-Mouse

• Cross Reactivity :

  Mouse

• Target Antigen :

  Recombinant mouse protein GDF-5

• Clone :

  (#13J15)

• Applications :

  WB, N

• Purification Method :

  Protein G/A chromatography

• Assay Protocol :

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form :

  Lyophilized

• Buffer :

  PBS

• Reconstitution :

  PBS

• Storage Conditions :

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source :

  Rat

• Isotype :

  IgG2