Gas-1

Gas1 (Growth Arrest Specific 1) is one of six structurally unrelated proteins that were identified by their increased expression in growth-arrested cells relative to actively proliferating cells. Following mitogenic stimulation, Gas1 expression is transcriptionally suppressed by c-Myc as cells transit from G0 to G1 phases of the cell cycle. Overexpression of Gas1 prevents S phase entry and DNA synthesis. Gas1-mediated blockade of the cell cycle is p53-dependent but does not require the transactivating domain of p53. The human Gas1 cDNA encodes a 345 amino acid (aa) precursor that includes a 39 aa signal sequence, a 279 aa mature protein, and a 27 aa C-terminal propeptide. Gas1 contains Ala-rich and Asp-rich regions as well as an RGD sequence. Mature human and mouse Gas1 share 85% aa sequence identity. Human Gas1 is a 40 kDa GPI-linked glycoprotein that is uniformly distributed on the cell surface. In contact-inhibited vascular endothelial cells, Gas1 is induced by VE-Cadherin and VEGF expression and mediates the anti-apoptotic effect of VEGF.

Growth arrest-specific protein 1

2619

P54826

NP_002039.2

NM_002048.2

Anti-Human

Human

Recombinant human Gas-1

(#3H43)

WB, Capture Ab

Protein G chromatography

Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Lyophilized

PBS

PBS

Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.

Rat