Eotaxin-2

• Description :

  Eotaxin2, also named MPIF-2 and Ckβ6, is a CC chemokine that is designated CCL24. Eotaxin2 cDNA encodes a 10.5 kDa, 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. C-terminally truncated variants with 78, 73, 75 and 76 residues have also been described. Eotaxin 2 shares 40%, 42% and 39% amino acid sequence identity with other CC chemokines CCL7/MCP3, CCL3/MIP1α, and CCL11/Eotaxin, respectively. Eotaxin2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant Eotaxin2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes, but not monocytes or activated T lymphocytes. Eotaxin2 also suppresses colony formation by high proliferative multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin2, Eotaxin and CCL13/MCP4 are mediated by the receptor CCR3.

• Synonyms :

  CCL24; Ckb-6; MPIF2; MPIF-2; SCYA24

• NCBI Gene ID :

  6369

• UniProt :

  O00175

• Accession Number :

  NP_002982.2

• Accession Number mRNA :

  NM_002991

• Chromosomal Location :

  7q11.23

• Reactivity :

  Anti-Human

• Cross Reactivity :

  Human

• Target Antigen :

  Recombinant Human Eotaxin-2 (CCL24)

• Applications :

  ELISA, WB, IHC (P)

• Purification Method :

  Protein A chromatography

• Assay Protocol :

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Form :

  Lyophilized from PBS

• Buffer :

  PBS

• Reconstitution :

  Water

• Storage Conditions :

  The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.

• Host or Source :

  Mouse

• Isotype :

  IgG1, kappa