Endocan/ESM-1

Endocan (endothelial cell proteoglycan), also known as endothelialcell specific molecule1 (ESM-1), is a 50 kDa monomeric, secreted, cysteinerich proteoglycan identified initially in endothelial cells of the kidney and lung. Mouse Endocan is synthesized as a 184 amino acid (aa) precursor that contains a 21 aa signal sequence and a 20 kDa, 163 aa mature region. The N-terminal 2/3 of the molecule contains 18 cysteine residues and there are no potential N-linked glycosylation sites. Based on human Endocan, there are at least two potential O-linked glycosylation sites, one of which will likely be utilized on Ser at position # 136 of the mature molecule. The posttranslational modification is approximately 30 kDa in size. It consists of a single dermatan sulfate chain that contains 4O sulfated N-acetyl galactosamine with αiduronate. This chain is suggested to bind HGF and contribute to HGF mitogenic activity. Mature mouse Endocan shares 96% and 74% aa identity with rat and human Endocan, respectively. In human, there is a potential for an alternate splice variant. It shows a deletion of aa 82-131, a range which would not remove the dermatan sulfate attachment site. It is not known if such a splice form exists in mouse. Endocan is expressed by endothelial cells, adipocytes, bronchial epithelium and distal renal tubular epithelium. It is upregulated by TNFα and VEGF, and is known to bind to LFA1 (integrin αLβ2) on the surface of PBMCs, blocking LFA1 interaction with ICAM1. Normal circulating levels of Endocan are approximately 1 ng/mL.

Esm1; ESM-1; AV004503; 0610042H23Rik

71690

Q9QYY7

NP_076101.1

NM_023612.3

13 D2.2; 13

Anti-Mouse

Mouse

Recombinant mouse ESM-1 (RT #M30-062)

Rabbit IgG

WB

Protein A purified

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Western Blot: use at 1-5 µg/ml.

Lyophilized

PBS

Water

The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.