CEACAM-1

• Description:

  Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM-1), also known as CD66a and BGP-I, is a 115 kDa type I transmembrane glycoprotein that belongs to the membrane-bound CEA subfamily of the CEA superfamily. On the surface of cells, it forms noncovalent homo- and heterodimers. The extracellular region contains three C2-type Ig-like domains and one N-terminal V-type Ig-like domain. Multiple splice variants involving regions C-terminal to the second C2-type domain (aa 320 and beyond) exist.

• Synonyms:

  CEACAM1; BGP; BGP1; BGPI, CD66a

• NCBI Gene ID:

  634

• UniProt:

  P13688

• Accession Number:

  NP_001192273.1

• Accession Number mRNA:

  NM_001205344.1

• Chromosomal Location:

  19q13.2

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Recombinant human soluble CEACAM-1-Fc (produced in HEK293 cells)

• Clone:

  (#C5-1X)

• Applications:

  WB, IHC, E, IF, IP, FC

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  ELISA: Use at 10 µg/ml; WB: Use at 10 µg/ml; Immunohistochemistry: Use at 10 µg/ml; FACS analysis: Use at 10 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Notes:

  Anti-human CEACAM-1 (C5-1X) interacts solely with human CEACAM-1 and shows no cross-reactivity with other CEACAMs expressed in human or other species!

• Host or Source:

  Mouse

• Isotype:

  IgG1, kappa