ACE

• Description :

  Angiotensin I Converting Enzyme (ACE, also known as peptidyl-dipeptidase A, kininase II, or CD143) is a type I transmembrane zinc metallopeptidase that is important in the metabolism of the vaso-active peptides angiotensin II and bradykinin. Two isoforms of ACE that exhibit distinct physiologic functions have been described: germinally expressed ACE (gACE) and somatic cell expressed ACE (sACE) . Both isoforms are transcribed from a single gene using alternative promoters. The gACE isoform is exclusively expressed in the testes, whereas sACE is expressed on endothelial cells, epithelial cells, neuronal cells, and dendritic cells. A soluble form of CD143 is likely shed from endothelial cells and is found in serum, plasma, seminal fluid, amniotic fluid and cerebral spinal fluid. Functionally, CD143 has a role in blood pressure regulation, electrolyte balance, reproduction, cardiovascular disease, kidney disease, cancer and Alzheimer’s disease. Inhibitors of ACE are used clinically to treat hypertension, cardiac failure, and diabetic retinopathy.

• Synonyms :

  Angiotensin I converting enzyme; CD143; testicular ECA carboxycathepsin; DCP; ACE1; DCP1; MVCD3

• NCBI Gene ID :

  1636

• UniProt :

  P12821

• Accession Number :

  NP_00780.1

• Accession Number mRNA :

  NM_000789

• Chromosomal Location :

  17q23.3

• Reactivity :

  Anti-Human

• Cross Reactivity :

  Human

• Target Antigen :

  Human ACE recombinant protein (extracellular domain)

• Clone :

  (#12J16)

• Applications :

  WB

• Purification Method :

  Protein G chromatography

• Assay Protocol :

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Bioactivity :

  WB: 1:500-1000.

• Form :

  Lyophilized

• Buffer :

  PBS

• Reconstitution :

  PBS

• Storage Conditions :

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source :

  Mouse

• Isotype :

  IgG1