Rhesus macaque PD-1 / PDCD1 Protein, His Tag (MALS verified)

Rhesus macaque PD-1 / PDCD1 Protein, His Tag (MALS verified)

• Background: Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
• Description: Rhesus macaque PD-1, His Tag (PD1-R52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # B0LAJ2-1).
• Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 17.9 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Leu 25 - Gln 167
• Host: HEK 293
• Target: PD-1
• Conjugation: Unconjugated
• Tag: C-10×His
• Source: Rhesus macaque
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Rhesus macaque PD-1, His Tag (Cat. No. PD1-R52H3) at 2 μg/mL (100 μL/well) can bind nivolumab with a linear range of 0.1-6 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 17.9 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C