Recombinant murine Epidermal Growth Factor (rm EGF )

• Synonyms:

  Urogastrone, URG

• Source:

  Escherichia coli.

• Purity:

  > 97 % by SDS-PAGE and HPLC analyses.

• Bioactivity:

  Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.

• Molecular Weight:

  Approximately 6.0 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids, including 3 intramolecular disulfide-bonds.

• Storage Conditions:

  This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

• Formulation:

  Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

• CAS Number:

  9000-83-3