RegisterLogin

Alpha Synuclein Oligomers

CAT: 0400-SPR-484ESize: 5x 100 µgDry Ice: YesHazardous: No
CAT#:0400-SPR-484ESize:5x 100 µg
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Background
Our kinetically stable oligomers of alpha-synuclein are generated without an inducer or inhibitor and remain stable for at least 2 weeks at 37oC. They present as globular structures under TEM, demonstrate toxicity in rat primary dopaminergic neurons and induce Parkinson’s-associated alpha synuclein phosphoserine 129 pathology. These oligomers have been previously characterized as globular, cylindrical structures with a beta-sheet structure intermediate between monomers and fibrils, and were demonstrated to have a higher toxicity to neurons than alpha-synuclein fibrils (1,2) . Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (3) . Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (4) . Functionally, it has been shown to significantly interact with tubulin (5), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (6) . SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer’s disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (7, 8) .
Description
Human Recombinant Alpha Synuclein Oligomers (Kinetically Stable)
Product Name Alternative
Alpha synuclein pre-formed fibrils, Alpha synuclein aggregates, Alpha synuclein protein aggregates, Alpha synuclein aggregates, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, SYN protein, Parkison disease familial 1 Protein
UNSPSC
12352202
UN Code
Non-hazardous
Hazard Statement
Non-hazardous
Gene ID
6622
Swiss Prot
P37840
Accession Number
NP_000336.1
Cellular Locus
Cytoplasm | Cell Membrane | Nucleus | Presynaptic Termini
Expression System
E. coli
Host
E. coli
Origin Species
Human
Target
Alpha Synuclein Oligomers (Kinetically Stable)
Conjugation
No Tag
Nature
Recombinant
Sequence
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
Applications
WB | Native PAGE | In vivo assay | In vitro assay
Field of Research
Neuroscience | Neurodegeneration | Alzheimer's Disease | Tangles & Tau | Neuroscience | Neurodegeneration | Parkinson's Disease | Synuclein | Neuroscience | Neurodegeneration | Multiple System Atrophy
Purification Method
Ion-exchange Purified, monomer removed with 100K MWCO filter
Purification
Ion-exchange Purified, monomer removed with 100K MWCO filter
Limit Of Detection
Certified >95% pure using SDS-PAGE analysis. Low endotoxin <5 EU/mL @ 2mg/mL.
Concentration
2 mg/ml
Purity
>95%
Weight
0.05
Length
Full Length
Buffer
PB pH 7.4 (10 mM KH2PO4, 7.5 mM NaOH, pH 7.4)
Molecular Weight
650-1200 kDa
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.
Additionnal Information
Kinetically stable alpha synuclein oligomers are generated from monomer without an inducer/inhibitor, remain soluble oligomers for at least two weeks at 37 degrees, and are toxic to dopaminergic neurons.
References & Citations
1. Chen, S.W., et. al. (2015). PNAS. E1994-E2003. 2. Lorenzen, N., et. al. (2014). JACS. 136: 3859-3868. 3. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013). 4. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 5. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 6. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 7. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 8. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.
Shipping Conditions
Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.
Storage Conditions
-80ºC
Notes
Kinetically stable alpha synuclein oligomers are generated from monomer without an inducer/inhibitor, remain soluble oligomers for at least two weeks at 37 degrees, and are toxic to dopaminergic neurons.
Protein Length
Full Length
Background Reference 01
1. Chen, S.W., et. al. (2015) . PNAS. E1994-E2003. 2. Lorenzen, N., et. al. (2014) . JACS. 136: 3859-3868. 3. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013) . 4. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 5. Alim M.A., et al. (2002) J Biol Chem. 277 (3) : 2112-2117. 6. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231 (1) : 226-230. 7. Spillantini M.G., et al. (1997) Nature. 388 (6645) : 839-840. 8. Mezey E., et al. (1998) Nat Med. 4 (7) : 755-757.
Location
Cytoplasm | Cell Membrane | Nucleus | Presynaptic Termini
AA Sequence
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
Immunogen Species
Human
Product MSDS
Loading...

Related Products

CatalogName
SPR-323CAlpha Synuclein Monomers
SPR-316BAlpha Synuclein Monomers
SPR-316EAlpha Synuclein Monomers
SPR-450EAlpha Synuclein Filaments
SPR-450BAlpha Synuclein Filaments
SPR-321BAlpha Synuclein Monomers
SPR-481EAlpha Synuclein Monomers
SPR-321CAlpha Synuclein Monomers
SPR-321XEAlpha Synuclein Monomers
SPR-321EAlpha Synuclein Monomers

Alternative Products

CatalogName
MBS8006005-01Human Recombinant Alpha Synuclein Oligomers (Dopamine HCL Stabilized)
MBS8006006-01Human Recombinant Alpha Synuclein Oligomers (Epigallocatechin gallate (EGCG) Stabilized)
NBP3-14772-200ugRecombinant Human alpha-Synuclein Oligomers, (Dopamine HCL Stabilized) Protein - (Dry Ice)
216047Synuclein, alpha (Alpha-Synuclein)
367849Synuclein, alpha (alpha Synuclein, Synuclein) (Not for Export EU)
216969Synuclein, alpha (Synuclein-alpha, SNCA)
MBS8534935-01Alpha-Synuclein
052303Synuclein, alpha
528798-01Synuclein-alpha
MBS556581-01alpha Synuclein

Popular Products