Anti-Cyclin D1 Biotin

• Description:

  Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.

• Certification:

  RUO

• Negative Species:

  Cat, Sheep

• Reactivity:

  Rat, Mouse, Non-human primates, Human

• Immunogen:

  Recombinant human cyclin D1 (amino acids 1-295)

• Target Antigen:

  Cyclin D1

• Clone:

  DCS-6

• Applications:

  IHC-F, IP, ICC, IHC-P, WB, FC-IC

• Concentration:

  1 mg/mL

• Format:

  Biotin

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Bosone I, Cavalla P, Chiadò -Piat L, Vito ND, Schiffer D: Cyclin D1 expression in normal oligodendroglia and microglia cells: its use in the differential diagnosis of oligodendrogliomas. Neuropathology. 2001 Sep;21 (3) :155-61. , URL: http://www.ncbi.nlm.nih.gov/pubmed/11666011 , *Jain P, Giustolisi GM, Atkinson S, Elnenaei MO, Morilla R, Owusu-Ankomah K, Rafiq-Mohammed F, Matutes E, Wotherspoon A, Catovsky D: Detection of cyclin D1 in B cell lymphoproliferative disorders by flow cytometry. J Clin Pathol. 2002 Dec;55 (12) :940-5., URL: http://www.ncbi.nlm.nih.gov/pubmed/12461064 , *Albrecht JH, Hansen LK: Cyclin D1 promotes mitogen-independent cell cycle progression in hepatocytes. Cell Growth Differ. 1999 Jun;10 (6) :397-404., URL: http://www.ncbi.nlm.nih.gov/pubmed/10392901 , *Zwijsen RM, Buckle RS, Hijmans EM, Loomans CJ, Bernards R: Ligand-independent recruitment of steroid receptor coactivators to estrogen receptor by cyclin D1. Genes Dev. 1998 Nov 15;12 (22) :3488-98., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=Zwijsen+rm+buckle+rs+bernards+r , *Fredersdorf S, Burns J, Milne AM, Packham G, Fallis L, Gillett CE, Royds JA, Peston D, Hall PA, Hanby AM, Barnes DM, Shousha S, O'Hare MJ, Lu X: High level expression of p27 (kip1) and cyclin D1 in some human breast cancer cells: inverse correlation between the expression of p27 (kip1) and degree of malignancy in human breast and colorectal cancers. Proc Natl Acad Sci U S A. 1997 Jun 10;94 (12) :6380-5., URL: https://pubmed.ncbi.nlm.nih.gov/9177226/

• Other References:

  *Diehl JA, Zindy F, Sherr CJ: Inhibition of cyclin D1 phosphorylation on threonine-286 prevents its rapid degradation via the ubiquitin-proteasome pathway. Genes Dev. 1997 Apr 15;11 (8) :957-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/9136925?ordina, *Diehl JA, Cheng M, Roussel MF, Sherr CJ: Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization. Genes Dev. 1998 Nov 15;12 (22) :3499-511., URL: https://www.ncbi.nlm.nih.gov/pubmed/9832503?ordina, *Yang W, Zhang Y, Li Y, Wu Z, Zhu D: Myostatin induces cyclin D1 degradation to cause cell cycle arrest through a phosphatidylinositol 3-kinase/AKT/GSK-3 beta pathway and is antagonized by insulin-like growth factor 1. J Biol Chem. 2007 Feb 9;282 (6) :3799-808., URL: https://www.ncbi.nlm.nih.gov/pubmed/17130121?ordin, *Mukherji A, Janbandhu VC, Kumar V: GSK-3beta-dependent destabilization of cyclin D1 mediates replicational stress-induced arrest of cell cycle. FEBS Lett. 2008 Mar 6, URL: https://www.ncbi.nlm.nih.gov/pubmed/18328826?ordin

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The mouse monoclonal antibody DCS-6 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.

• Applications Notes:

  Flow cytometry: Recommended dilution: 2-10 μg/ml. Intracellular staining.Immunohistochemistry: Recommended dilution: 5-10 μg/ml.

• Isotype:

  Mouse IgG2a