Anti-Cyclin B1 Purified

Anti-Cyclin B1 Purified

• Background: Cyclin B1 is a regulatory protein involved in mitosis. It complexes with p34(cdc2) to form maturation-promoting factor (MPF), and is necessary for proper control of the G2/M phase transition of the cell cycle. It is expressed in tissues containing proliferating cells, such as lymph node, testis et al.
• Specifications: The mouse monoclonal antibody V152 recognizes cyclin B1, a 48 kDa intracellular protein necessary for G2/M phase transition of the cell cycle.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Mammalian
• Immunogen: Recombinant His-tagged hamster cyclin B1
• Target Antigen: Cyclin B1
• Isotype: IgG1
• Clone: V152
• Applications: Flow cytometry: Recommended dilution: 2-5 µg/mL. Intracellular staining.
• Validated Applications: WB, IHC-P, ICC, FC, IHC-F
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Yang Y, Xue K, Li Z, Zheng W, Dong W, Song J, Sun S, Ma T, Li W: c-Myc regulates the CDK1/cyclin B1 dependent‑G2/M cell cycle progression by histone H4 acetylation in Raji cells. Int J Mol Med. 2018 Jun41(6):3366-3378., URL: https://www.ncbi.nlm.nih.gov/pubmed/29512702,*Wolf F, Wandke C, Isenberg N, Geley S: Dose-dependent effects of stable cyclin B1 on progression through mitosis in human cells. EMBO J. 2006 Jun 2125(12):2802-13., URL: https://www.ncbi.nlm.nih.gov/pubmed/16724106,*Balsara ZR, Misaghi S, Lafave JN, Starnbach MN: Chlamydia trachomatis infection induces cleavage of the mitotic cyclin B1. Infect Immun. 2006 Oct74(10):5602-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/16988235,*Geley S, Kramer E, Gieffers C, Gannon J, Peters JM, Hunt T: Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint. J Cell Biol. 2001 Apr 2153(1):137-48., URL: https://pubmed.ncbi.nlm.nih.gov/11285280/,*Chan YW, Chen Y, Poon RY: Generation of an indestructible cyclin B1 by caspase-6-dependent cleavage during mitotic catastrophe. Oncogene. 2009 Jan 1528(2):170-83., URL: https://www.ncbi.nlm.nih.gov/pubmed/18820706,
• Storage Conditions: Store at 2-8°C. Do not freeze.