Anti-Hu CD335 PE

• Description:

  CD335 is a type I transmembrane glycoprotein, which serves as one of natural cytotoxicity receptors, and represents major receptor informing NK cells that they should kill particular virus-infected or tumor cell. Its sialic acids residues are involved in recognition of viral hemagglutinins, similarly it recognizes heparan sulphate proteoglycans on the surface of tumor cells. CD335 transfers the signal downstream via association with CD3 zeta chain, that contains intracellular ITAM motifs. Expression of CD335, a specific marker of NK cells (both resting and activated), is down regulated by cortisol, and up-regulated by prolactin.

• Certification:

  RUO

• Immunogen:

  Fusion protein of human CD335 and Fc part of IgG

• Target Antigen:

  CD335

• Clone:

  9E2

• Applications:

  FC (QC tested)

• Format:

  PE

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Durrenberger PF, Ettorre A, Kamel F, Webb LV, Sim M, Nicholas RS, Malik O, Reynolds R, Boyton RJ, Altmann DM: Innate immunity in multiple sclerosis white matter lesions: expression of natural cytotoxicity triggering receptor 1 (NCR1) . J Neuroinflammation. 2012 Jan 2;9:1. doi: 10.1186/1742-2094-9-1., URL: https://pubmed.ncbi.nlm.nih.gov/22212381/, *Alcántara-Hernández M, Leylek R, Wagar LE, Engleman EG, Keler T, Marinkovich MP, Davis MM, Nolan GP, Idoyaga J: High-dimensional phenotypic mapping of human dendritic cells reveals interindividual variation and tissue specialization. Immunity. 2017 Dec 19;47 (6) :1037-1050.e6., URL: https://pubmed.ncbi.nlm.nih.gov/29221729/, *Strittmatter-Keller U, Walter C, Rauld C, Egli N, Regairaz C, Rabe S, Zenke G, Carballido J, Schweighoffer T: Fingerprints of CD8+ T cells on human pre-plasma and memory B cells. PLoS One. 2018 Dec 12;13 (12) :e0208187., URL: https://pubmed.ncbi.nlm.nih.gov/30540814/, *Konnikova L, Boschetti G, Rahman A, Mitsialis V, Lord J, Richmond C, Tomov VT, Gordon W, Jelinsky S, Canavan J, Liss A, Wall S, Field M, Zhou F, Goldsmith JD, Bewtra M, Breault DT, Merad M, Snapper SB: High-dimensional immune phenotyping and transcriptional analyses reveal robust recovery of viable human immune and epithelial cells from frozen gastrointestinal tissue. Mucosal Immunol. 2018 Nov;11 (6) :1684-1693., URL: https://pubmed.ncbi.nlm.nih.gov/30111863/, *Frutoso M, Mair F, Prlic M: OMIP-070: NKp46-Based 27-color phenotyping to define natural killer cells isolated from human tumor tissues. Cytometry A 2020 Sep 26. doi: 10.1002/cyto.a.24230. , URL: https://pubmed.ncbi.nlm.nih.gov/32978859/

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody 9E2 recognizes an extracellular epitope of human CD335, a transmembrane glycoprotein, serving as a natural cytotoxicity receptor against virus-infected or cancer cells.

• Applications Notes:

  6

• Species:

  Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1