Anti-Hu CD370 APC

• Description:

  CD370 / CLEC9A, also known as DNGR1, is a type II transmembrane glycoprotein with extracellular C-type lectin domain and intracellular ITAM-containing domain. Its expression is restricted to BDCA3+ conventional dendritic cells and to a subset of CD14+ CD16- monocytes. CD370 serves as a receptor for ubiquitous preformed acid-labile protein associated ligands that are exposed when the cell membrane is damaged, such as on necrotic cells. Its triggering by these ligands mediates recruitment and activation of the tyrosine kinase Syk and leads to their cross-presentation to the immune system.

• Certification:

  RUO

• Reactivity:

  Human

• Immunogen:

  RBL-2H3 cells expressing human CLEC9A fused to an HA epitope

• Target Antigen:

  CD370

• Clone:

  8F9

• Applications:

  FC (QC tested)

• Format:

  APC

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Sancho D, Mourã o-Sá D, Joffre OP, Schulz O, Rogers NC, Pennington DJ, Carlyle JR, Reis e Sousa C: Tumor therapy in mice via antigen targeting to a novel, DC-restricted C-type lectin. J Clin Invest. 2008 Jun;118 (6) :2098-110. doi: 10.1172/JCI34584., URL: http://www.ncbi.nlm.nih.gov/pubmed/18497879 , *Poulin LF, Reyal Y, Uronen-Hansson H, Schraml BU, Sancho D, Murphy KM, Hå kansson UK, Moita LF, Agace WW, Bonnet D, Reis e Sousa C: DNGR-1 is a specific and universal marker of mouse and human Batf3-dependent dendritic cells in lymphoid and nonlymphoid tissues. Blood. 2012 Jun 21;119 (25) :6052-62., URL: http://www.ncbi.nlm.nih.gov/pubmed/22442345 , *Segura E, Valladeau-Guilemond J, Donnadieu MH, Sastre-Garau X, Soumelis V, Amigorena S: Characterization of resident and migratory dendritic cells in human lymph nodes. J Exp Med. 2012 Apr 9;209 (4) :653-60., URL: http://www.ncbi.nlm.nih.gov/pubmed/22430490

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody 8F9 recognizes an extracellular epitope of CD370 / CLEC9A (DNGR1), a type II transmembrane protein functioning as an endocytic receptor on BDCA31+ dendritic cells and on a subset of CD14+ CD16- monocytes.

• Applications Notes:

  6

• Isotype:

  Mouse IgG2a