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Anti-GRAP2 APC

CAT: 0270-1A-639-C100Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0270-1A-639-C100Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GRAP2/GADS (Grb2-related adaptor protein 2 / Grb2-related adaptor downstream of Shc) is a cytoplasmic adaptor protein containing N- and C-terminal SH3 domains flanking a central SH2 domain and a proline/glutamine-rich region. It is expressed predominantly in lymphoid tissue and hematopoietic cells, particularly in T cells. GRAP2/GADS plays a pivotal role during the early events of T cell signal transduction by recruiting the adaptor protein SLP-76 and its associated molecules, such as Vav, Nck, Itk, and ADAP, to the transmembrane adaptor protein LAT. GRAP2/GADS also binds several other signaling proteins, namely Gab2, HPK1 (hematopoietic progenitor kinase 1), and Cbl. Unlike similar adaptor protein Grb2, GRAP2/GADS shows higher selectivity when binding to the particular phosphorylated tyrosines of LAT adaptor.
Certification
RUO
Reactivity
Human
Immunogen
GST-fusion human GRAP2/GADS protein
Target Antigen
GRAP2
Clone
UW40
Applications
FC-IC (QC tested)
Concentration
0.1 mg/mL
Format
APC
Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Yankee TM, Yun TJ, Draves KE, Ganesh K, Bevan MJ, Murali-Krishna K, Clark EA: The Gads (GrpL) adaptor protein regulates T cell homeostasis. J Immunol. 2004 Aug 1;173 (3) :1711-20., URL: http://www.ncbi.nlm.nih.gov/pubmed/15265900 , *Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15 (11) :909-912., URL: https://www.ncbi.nlm.nih.gov/pubmed/30377371 , *Yankee TM, Draves KE, Ewings MK, Clark EA, Graves JD: CD95/Fas induces cleavage of the GrpL/Gads adaptor and desensitization of antigen receptor signaling. Proc Natl Acad Sci U S A. 2001 Jun 5;98 (12) :6789-93., URL: http://www.ncbi.nlm.nih.gov/pubmed/11391000
Other References
*Watanabe R, Harada Y, Takeda K, Takahashi J, Ohnuki K, Ogawa S, Ohgai D, Kaibara N, Koiwai O, Tanabe K, Toma H, Sugamura K, Abe R: Grb2 and Gads exhibit different interactions with CD28 and play distinct roles in CD28-mediated costimulation. J Immunol. 2006 Jul 15;177 (2) :1085-91., URL: https://www.ncbi.nlm.nih.gov/pubmed/16818765, *Moran O, Roessle MW, Mariuzza RA, Dimasi N: Structural features of the full-length adaptor protein GADS in solution determined using small-angle X-ray scattering. Biophys J. 2008 Mar 1;94 (5) :1766-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/17993503, *Dalheimer SL, Zeng L, Draves KE, Hassaballa A, Jiwa NN, Parrish TD, Clark EA, Yankee TM: Gads-deficient thymocytes are blocked at the transitional single positive CD4+ stage. Eur J Immunol. 2009 May;39 (5) :1395-404., URL: https://www.ncbi.nlm.nih.gov/pubmed/19337995, *Cho S, Velikovsky CA, Swaminathan CP, Houtman JC, Samelson LE, Mariuzza RA: Structural basis for differential recognition of tyrosine-phosphorylated sites in the linker for activation of T cells (LAT) by the adaptor Gads. EMBO J. 2004 Apr 7;23 (7) :1441-51, URL: https://www.ncbi.nlm.nih.gov/pubmed/15029250
Storage Conditions
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Specificity
The mouse monoclonal antibody UW40 recognizes GRAP2/GADS, a 41 kDa cytoplasmic adaptor protein that plays a pivotal role during the early events of signal transduction in T cells.
Applications Notes
Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
Isotype
Mouse IgG2a

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